By D. G. Hardie (Editor)
Reversible phosphorylation is among the significant mechanisms of controlling protein task in all eukaryotic cells. This new version of Protein Phosphorylation: a pragmatic procedure presents a complete description of present tools used to check protein phosphorylation and the kinases and phosphatases which catalyse it. It comprises protocols for learning phosphorylation in intact cells; research of sign transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and id of substrates. additionally lined are cloning and expression protocols and suggestion at the crystallization of kinases and phosphatases. Protein Phosphorylation: a pragmatic procedure 2e will consequently be of serious price to any researcher investigating points of reversible protein phosphorylation.
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